Citation

Salvador E, Burek M, Förster CY. Methods Mol. Biol. 2018; 1717: 219-227.

Affiliation

Department of Anaesthesia and Critical Care, University of Wuerzburg, Wuerzburg, Germany. Foerster_C@ukw.de.

Copyright

(Copyright © 2018, Holtzbrinck Springer Nature Publishing Group)

DOI

10.1007/978-1-4939-7526-6_17

PMID

29468595

Abstract

Traumatic brain injury (TBI) is a significant problem causing high mortality globally.

METHODS to increase possibilities for treatment and prevention of secondary injuries resulting from the initial physical insult are thus much needed. TBI affects the central nervous system (CNS) and the neurovascular unit as a whole in numerous ways but one of the primarily compromised components is the blood-brain barrier (BBB).In this chapter, we present a detailed procedure on how stretch injury and oxygen-glucose deprivation (OGD) are applied to brain microvascular endothelial cells of the BBB in order to replicate the actual impact they receive during TBI.

Language: en

Keywords

In vitro model; Murine cerebrovascular endothelial cells; Stretch injury; Traumatic brain injury; cEND