Citation

Chu Y, Han GX, Wang YQ, Shan HY, Chen XP, Tao LY, Zhang MY. Fa Yi Xue Za Zhi 2017; 33(3): 221-224.

Affiliation

Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.

Copyright

(Copyright © 2017, Si fa bu Si fa jian ding ke xue ji shu yan jiu suo)

DOI

10.3969/j.issn.1004-5619.2017.03.001

PMID

29230982

Abstract

OBJECTIVES: To observe the changes of cystathionine β-synthase （CBS） expression in the cerebral cortex after brain contusion at different times.

METHODS: An experimental model of traumatic brain injury （TBI） in mice was established by an improved weight-drop device. Then Western blotting and immunohistochemical examination were used to detect the CBS expression in cerebral cortex around injury at different time points （1 h, 6 h, 12 h, 1 d, 2 d, 3 d, 7 d）.

RESULTS: The results of Western blotting revealed that the expression level of CBS was down-regulated and reached its lowest level at the 3rd days after injury, and then restored to normal level after 7 days. The results of immunohistochemistry showed that CBS was present in the normal brain cortex. CBS expression gradually decreased at the 3rd days after injury, and then restored to normal level after 7 days.

CONCLUSIONS: CBS has the potential to be a reference index for time estimation after brain contusion in forensic practice.

Language: zh

Keywords

brain injuries; cystathionine beta-synthase; forensic pathology; injury time; mice