Citation

Colombo G, Lobina C, Lorrai I, Acciaro C, Maccioni P, Gessa GL. Alcohol 2017; 63: 27-32.

Affiliation

Neuroscience Institute, Section of Cagliari, National Research Council of Italy, S.S. 554, Km. 4,500, I-09042, Monserrato, CA, Italy.

Copyright

(Copyright © 2017, Elsevier Publishing)

DOI

10.1016/j.alcohol.2017.04.002

PMID

28847379

Abstract

Previous studies suggested that exposure of Sardinian alcohol-preferring (sP) rats to daily drinking sessions of 1 h, during the dark phase of the light/dark cycle, with multiple alcohol concentrations, and unpredictable access to alcohol, resulted in exceptionally high intakes of alcohol when the drinking session occurred over the last hours of the dark phase. Additionally, higher levels of anxiety-related behaviors were observed at the 12th, rather than 1st, hour of the dark phase, suggesting that uncertainty of time of alcohol access and expectation of alcohol availability produced an emotional "distress". The present study was designed to provide pharmacological support to the hypothesis that high alcohol intake under this drinking procedure is secondary to exacerbation of the anxiety-like state of sP rats. To this end, sP rats were initially exposed to daily 1-h drinking sessions during the dark phase and with multiple alcohol concentrations (0%, 10%, 20%, and 30%; v/v); time of alcohol exposure was changed each day and was unpredictable to rats. Rats were then treated acutely with non-sedative doses of diazepam (0, 1, 2, and 3 mg/kg; intraperitoneally [i.p.]) before two drinking sessions occurring at the 1st and 12th hour of the dark phase, respectively. Treatment with diazepam was ineffective at the 1st hour; conversely, it selectively reduced alcohol intake (up to 50% at the dose of 3 mg/kg) at the 12th hour. The preferential effectiveness of diazepam in reducing alcohol intake when the drinking session occurred at the 12th hour of the dark phase is consistent with the hypothesis that uncertainty of time of alcohol access and expectation of alcohol availability generated an emotional "distress" that rats counterbalanced with high alcohol drinking; the results of the present study are interpreted as the anxiolytic effects of diazepam substituting for those of alcohol, resulting in the observed reduction in alcohol intake.

Copyright © 2017 Elsevier Inc. All rights reserved.

Language: en

Keywords

Anxiety-like behaviors; Diazepam; Experimental model of binge drinking; Limited and unpredictable access to alcohol; Sardinian alcohol-preferring (sP) rats; Time schedule of alcohol drinking