Citation

Cardinell BA, La Belle JT. Methods Mol. Biol. 2017; 1572: 89-112.

Affiliation

School of Medicine, Mayo Clinic, Scottsdale, AZ, USA. Jeffrey.LaBelle@asu.edu.

Copyright

(Copyright © 2017, Holtzbrinck Springer Nature Publishing Group)

DOI

10.1007/978-1-4939-6911-1_7

PMID

28299683

Abstract

Electrochemical detection methods have been popular in the medical diagnostics field. Several well-known devices such as the self-monitoring blood glucose meter have relied on electrochemical techniques for their sensitivity, and ability to make direct measurements without optical labels. Currently, no point-of-care or handheld diagnostic tool exists to quantify the severity of a traumatic brain injury (TBI). We have shown that enzymatic detection of norepinephrine (NE), a biomarker which can indicate TBI severity, using impedance-based electrochemical techniques can achieve the required sensitivity, ∼100 pg/mL. Furthermore, the first steps have been taken to quantify NE in whole blood solutions and to optimize the technique for a handheld device.

Language: en

Keywords

Biomarker; Biosensor; Electrochemical impedance spectroscopy; Enzyme; Impedance time; Norepinephrine; Point-of-care technology; Traumatic brain injury