Citation

Mirshamsi MR, Omranipour R, Vazirizadeh A, Fakhri A, Zangeneh F, Mohebbi GH, Seyedian R, Pourahmad J. Asian Pac. J. Cancer Prev. 2017; 18(1): 277-286.

Affiliation

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Email: j.pourahmadjaktaji@utoronto.ca

Copyright

(Copyright © 2017, National Cancer Center, Korea, Publisher Asian Pacific Organization for Cancer Prevention)

DOI

10.22034/APJCP.2017.18.1.277

PMID

28240847

Abstract

OBJECTIVE: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas.

METHODS: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of caspase3 and apoptosis were then assayed.

RESULT: Our results demonstrate that fraction 2 of Cassiopea andromeda crude venom significantly (P<0.05) decreased mitochondrial succinate dehydrogenase activity, increased mitochondrial ROS production, induced mitochondrial swelling, MMP collapse and cytochrome c release, activated caspase3 and induced apoptosis only in tumoral mitochondria, and not in mitochondria obtained from extra-tumoral tissue (P<0.05).

CONCLUSION: In conclusion this study suggested that fraction 2 of Cassiopea andromeda crude venom selectively induces ROS mediated cytotoxicity by directly targeting mitochondria isolated from human breast tumor tissue.

Creative Commons Attribution License

Language: en

Keywords

Cassiopea andromeda venom; gel filtration; selective toxicity; Isolated mitochondria; Apoptosis