Citation

Zander NE, Piehler T, Hogberg H, Pamies D. Cell. Mol. Neurobiol. 2017; 37(7): 1331-1334.

Affiliation

Center for Alternatives to Animal Testing, Johns Hopkins University, Bloomberg School of Public Health, 615N. Wolfe Street, Baltimore, MD, 21205, USA.

Copyright

(Copyright © 2017, Holtzbrinck Springer Nature Publishing Group)

DOI

10.1007/s10571-017-0463-7

PMID

28110483

Abstract

The effects of primary explosive blast on brain tissue still remain mostly unknown. There are few in vitro models that use real explosives to probe the mechanisms of injury at the cellular level. In this work, 3D aggregates of human brain cells or brain microphysiological system were exposed to military explosives at two different pressures (50 and 100 psi).

RESULTS indicate that membrane damage and oxidative stress increased with blast pressure, but cell death remained minimal.

Language: en

Keywords

Explosives; In vitro model; Induced pluripotent stem cells; Minibrain; Primary blast; Traumatic brain injury