|
Citation
|
Khedir SB, Bardaa S, Chabchoub N, Moalla D, Sahnoun Z, Rebai T. Pharm. Biol. 2016; 55(1): 1407-1414. |
|
Affiliation
|
Laboratory of Histology, Embryology and Reproductive Biology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia ; |
|
Abstract
|
CONTEXT: Since antiquity, Pistacia lentiscus L. (Anacardiaceae) fruit oil (PLFO) has been used as a remedy for primary health care such as burn treatment.
OBJECTIVE: This study assesses the healing effect of PLFO on CO2 laser fractional burn in a rat model.
MATERIALS AND METHODS: The study was carried out on 18 adult male Wistar rats. A second-degree laser burn (wound area = 2.2 cm(2)) was inflicted in the dorsal region by the application of CO2 fractional laser within the following parameters; Energy level: 25 MJ and Depth level: 4. After applying laser, the rats were divided into three groups: the first was treated with saline solution, the second with a reference cream 'CYTOL BASIC(®)' (0.13 μg/mm(2)) and the third with PLFO (0.52 μL/mm(2)). All treatments were topically administered for eight days. The healing effect was assessed using macroscopic, histological and biochemical parameters.
RESULTS: After eight days, the higher percentage of wound healing contraction was observed among the PLFO-treated group (100%) followed by the 'CYTOL BASIC(®)' treated group (61.36%) and untreated group (32.27%). During the treatment, the PLFO-treated group showed less erythema, less crusting/scabbing, higher general wound appearance scores and a high content of collagen (220.67 ± 7.48 mg/g of tissue) than the other groups.
DISCUSSION AND CONCLUSION: The current study has shown, for the first time, the healing effect of PLFO on CO2 laser fractional burn. Their wound healing effect could be attributed to their antimicrobial, anti-inflammatory and antioxidant effects.
Language: en |