Citation

Barrientos LS, Crespi JA, Fameli A, Posik DM, Morales H, Peral García P, Giovambattista G. Leg. Med. (Elsevier) 2016; 22: 54-57.

Affiliation

IGEVET - Instituto de Genética Veterinaria (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, UNLP, La Plata, Buenos Aires, Argentina. Electronic address: ggiovam@fcv.unlp.edu.ar.

Copyright

(Copyright © 2016, Japanese Society of Legal Medicine, Publisher Elsevier Publishing)

DOI

10.1016/j.legalmed.2016.08.002

PMID

27591540

Abstract

Dog fecal samples were collected at the crime scene and from the shoes of the suspect to see whether they could be linked. DNA was genotyped using a 145bp fragment containing a 60bp hotspot region of the mitochondrial DNA (mtDNA) control region. Once the species origin was identified, sequences were aligned with the 23 canine haplotypes defined, showing that evidence and reference had 100% identity with haplotype 5. The frequency of haplotype 5 and the exclusion power of the reference population were 0.056 and 0.89, respectively. The forensic index showed that it was 20 times more likely that the evidence belonged to the reference dog than to some other unknown animal. The results support that the mtDNA hypervariable region 1 (HV1) is a good alternative for typing in trace or degraded casework samples when the STR panel fails, and demonstrate the utility of domestic animal samples to give additional information to solve human legal cases.

Copyright © 2016. Published by Elsevier Ireland Ltd.

Language: en