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Citation |
Nan L, Qing S, Hui WJ, Hui HC, Rong J, Qin LF, Tao J. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess. 2015; 32(12): 2148-2153. |
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Affiliation |
a Key Laboratory of Food Safety Risk Assessment, Ministry of Health , China National Center for Food Safety Risk Assessment , Beijing , China. |
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Copyright |
(Copyright © 2015, Informa - Taylor and Francis Group) |
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DOI |
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PMID |
26413972 |
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Abstract |
The purpose of this study was to identify the pufferfish species and detect the tetrodotoxin (TTX) in roasted fish fillet samples which were collected in Beijing, Qingdao and Xiamen of China. Cytochrome c oxidase I (COI) gene was used as target gene for identification of the pufferfish species in samples. Enzyme-linked immunosorbent assay (ELISA) were used to screen the TTX levels in samples which had been detected as containing pufferfish by DNA barcode. 125 samples were identified by DNA barcodes, 32(26%) samples contained pufferfish composition and among them 26 (81%) were the highly toxic species named Lagocephalus lunaris. All 32 samples containing the pufferfish composition were positive for TTX with the levels ranging from 100 ng/g to 63,800 ng/g. Most of the 32 samples contained the highly toxic species Lagocephalus lunari. Based on the results, we suggest that the monitoring of roasted fish fillet should be strengthened and the processing procedures should be standardised to minimise TTX poisoning caused by pufferfish. Language: en |