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Citation |
Rønning HT, Madslien EH, Asp TN, Granum PE. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess. 2015; 32(12): 2120-2130. |
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Affiliation |
School of veterinary science , Norwegian University of life sciences , Oslo , Norway. |
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Copyright |
(Copyright © 2015, Informa - Taylor and Francis Group) |
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DOI |
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PMID |
26414385 |
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Abstract |
Lichenysin which is produced by 53 different Bacillus licheniformis strains has been structurally examined with a qualitative liquid chromatography - tandem mass spectrometry (LC-MS/MS) method using quadrupole - time of flight mass spectrometry. The same lichenysin isoforms are produced from all strains, indicating that the growth conditions have a stronger influence on the lipopeptide production than the genotype. A rapid method for the quantification of lichenysin from bacterial cell cultures with LC-MS/MS after a simple methanol extraction has been refined. For the first time, commercially available lichenysin has been used as calibrant, making quantification more accurate. The trueness for C15-lichenysin has been improved to 94% using matrix-matched calibration with lichenysin compared to 30% using solvent calibration with surfactin. The quantitative method was fully validated based on Commission Decision 2002/657/EC. The LOD of the method was below 1 µg g(-1) and the repeatability ranged from 10% to 16%. Language: en |