Citation

Mariotti Kde C, Schuh RS, Ferranti P, Ortiz RS, Souza DZ, Pechansky F, Fröehlich PE, Limberger RP. J. Anal. Toxicol. 2014; 38(7): 432-437.

Affiliation

Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Laboratório de Análises e Pesquisas Toxicológicas, Universidade Federal do Rio Grande do Sul, 2752 Ipiranga Avenue, Santana, Porto Alegre, Rio Grande do Sul 90610-000, Brazil.

Copyright

(Copyright © 2014, Preston Publications)

DOI

10.1093/jat/bku063

PMID

25038769

Abstract

Brazil is considered one of the countries with the highest number of amphetamine-type stimulant (ATS) users worldwide, mainly diethylpropion (DIE) and fenproporex (FEN). The use of ATS is mostly linked to diverted prescription stimulants and this misuse is widely associated with (ab)use by drivers. A validated method was developed for the simultaneous analysis of amphetamine (AMP), DIE and FEN in plasma samples employing direct immersion-solid-phase microextraction, and gas chromatographic/mass spectrometric analysis. Trichloroacetic acid 10% was used for plasma deproteinization. In situ derivatization with propylchloroformate was employed. The linear range of the method covered from 5.0 to 100 ng/mL. The detection limits were 1.0 (AMP), 1.5 (DIE) and 2.0 ng/mL (FEN). The accuracy assessment of the control samples was within 85.58-108.33% of the target plasma concentrations. Recoveries ranged from 46.35 to 84.46% and precision was <15% of the value of relative standard deviation. This method is appropriate for screening and confirmation in plasma forensic toxicology analyses of these basic drugs.

Language: en