Citation

Funayama M, Aoki Y, Sebetan IM, Sagisaka K. Forensic Sci. Int. 1987; 34(3): 175-182.

Affiliation

Department of Forensic Medicine, Tohoku University School of Medicine, Sendai, Japan.

Copyright

(Copyright © 1987, Elsevier Publishing)

DOI

unavailable

PMID

3666621

Abstract

An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 microns in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.

Language: en