Citation

Weinmann W, Renz M, Pelz C, Brauchle P, Vogt S, Pollak S. Blutalkohol 1998; 35(3): 195-203.

Affiliation

Institut fur Rechtsmedizin, Klin. der Albert-Ludwigs-Universitat, 79104 Freiburg, Germany.

Copyright

(Copyright © 1998, International Committee on Alcohol, Drugs and Traffic Safety and Bund gegen Alkohol und Drogen im Straßenverkehr, Publisher Steintor Verlag)

DOI

unavailable

PMID

unavailable

Abstract

A GC/MS method for the simultaneous quantitation of amphetamine, benzoylecgonine, codeine and morphine using 0.1 mL serum has been developed. This method is based on a standard procedure for 1 mL serum samples (solid- phase extraction, derivatisation, GC/MS-analysis) which has now been modified for 0.1 mL serum-volumes. Linearity of calibration was achieved with standard GC/MS-conditions down to 1 to 5 ng/mL drug-concentration. For sample- preparation deuterated standards were added to spiked serum-samples, then solid-phase extraction and derivatisation of extracts with perfluoropropionic anhydride and pentafluoropropanol were performed. Analyses were carried out by means of GC/MS in Selected-Ion-Monitoring mode. The extraction efficiencies were between 86 and 98 % while the relative standard deviations of ten analyses were between 5.3 and 7.2 % (determined at a drug concentration of 10 ng/mL). With this method a GC/MS-analysis for verification of immunological results for amphetamine, benzoylecgonine, morphine and codeine can be achieved with as little as 0.1 mL serum-sample. However, this does not include tetrahydrocannabinol and its metabolites which cannot be detected by this method.