Citation

Schmitt G, Aderjan R, Kirchner M, Zimmer H. Blutalkohol 1998; 35(6): 428-433.

Affiliation

Rechtsmedizin/Verkehrsmedizin Inst., 69115 Heidelberg, Germany.

Copyright

(Copyright © 1998, International Committee on Alcohol, Drugs and Traffic Safety and Bund gegen Alkohol und Drogen im Straßenverkehr, Publisher Steintor Verlag)

DOI

unavailable

PMID

unavailable

Abstract

An ELISA was developed in analogy to the standard practice of immuno- chemical pre-testing of drugs and pharmaceutical substances. It was developed for the detection of ethyl glucuronide (EtG). The test kit's usefulness was examined using native sample materials as a screening test. This required the determination of EtG by means of ELISA using 22 anonymous urine samples as well as 15 serum samples, which were then compared with GC/MS results. In 7 cases deviations of below 30 % could be shown. False positive results could not be found. In 2 cases a false negative result could be found due to the high cut-off value. Measurements by means of ELISA tended to be slightly higher at a cut-off value of 1 and in 2 cases they even showed a false positive result with alcohol free samples. The pilot study presented an accuracy of approximately 71% and a sensitivity of 100 %. Accuracy could be improved by selection of antibodies.