Citation

Buijten JC, Rydberg U. Blutalkohol 1978; 15(1): 51-57.

Copyright

(Copyright © 1978, International Committee on Alcohol, Drugs and Traffic Safety and Bund gegen Alkohol und Drogen im Straßenverkehr, Publisher Steintor Verlag)

DOI

unavailable

PMID

unavailable

Abstract

A micro method, 20 (mu)l whole blood, for simultaneous determination of lactate, pyruvate, glucose and ethanol had been developed for an automated device, such as an AutoAnalyser. Those methods had shown to have a good reproducibility in a pooled blood sample. The S.D. expressed as a fraction of the mean was for lactate 0.5%, pyruvate 1.0%, lactate/pyruvate ratio 1.3%, glucose 1.0% and ethanol 0.7%. In the rat experiments the s(d) expressed as a fraction of the mean was for lactate 5.4%, pyruvate 5.2%, lactate/pyruvate ratio 5.3%, glucose 1.3% and ethanol 2.9%. The blood samples (20 (mu)l) were deproteinized with a modification of the Somogyi method, instead of sodium hydroxide we used ammonia for neutralization of the zinc sulphate solution.