Citation

Albermann ME, Musshoff F, Madea B. Anal. Bioanal. Chem. 2010; 396(7): 2441-2447.

Affiliation

Institute of Forensic Medicine, University Hospital Bonn, Stiftsplatz 12, 53111 Bonn, Germany. elena.albermann@uni-bonn.de

Copyright

(Copyright © 2010, Holtzbrinck Springer Nature Publishing Group)

DOI

10.1007/s00216-009-3388-2

PMID

20130844

Abstract

Hair analysis has become a powerful tool for the detection of chronic and past drug consumption. For several years, it has been possible to determine even the intake of ethanol in hair samples by detecting the ethanol metabolites ethyl glucuronide or fatty acid ethyl esters. Recently, new requirements were published for the use of EtG as an abstinence test (c(EtG) < 7 pg/mg) as well as for heavy-drinking detection (c(EtG) > 30 pg/mg). In order to perform abstinence tests, a sensitive LC-MS/MS procedure has been developed and fully validated according to the guidelines of forensic toxicology. The nine-point calibration curve showed linearity over the range of concentrations from 2-1,000 pg/mg. Detection and quantification limits were 1 and 4 pg/mg respectively. The intra- and inter-day precision and accuracy were always better than 20%. The validated procedure has successfully been applied to perform abstinence tests and to analyze hair samples from persons in withdrawal treatment. Concentrations between

Language: en