Citation

Ameno K, Fuke C, Ameno S, Kinoshita H, Ijiri I. J. Can. Soc. Forensic Sci. 1996; 29(2): 43-48.

Copyright

(Copyright © 1996, Canadian Society of Forensic Science, Publisher Informa - Taylor and Francis Group)

DOI

10.1080/00085030.1996.10757046

PMID

unavailable

Abstract

We describe here a simple and rapid detection of methamphetamine (MA) and amphetamine (AP) simultaneously in urine by gas chromatography (GC) using a solid-phase micro-extraction (SPME) technique. We used an SPME fiber (Polydimethylsiloxane thickness; 100μM) with a fiber holder for manual use. After addition of urine (1.0 ml), NaCI(0.5g) and 1-phenylbutylamine (5.0 μg, 1-PB, internal standard) into a vial (1.5 ml), we adjusted the sample to pH12 with NaOH (10N) and then rapidly sealed it with a teflon-coated septum. After immersion of the SPME fiber in the sample for 20 min, and then washing promptly in NaOH-H3BO4 buffer (pH12), the SPME needle was inserted into the injection port of the GC and the fiber extruded. Each peak of MA, AP and 1-PB was clearly separated. Linearity of AP and MA were in the ranges of 0.25-10 and 0.1-1.0 μg/ml, respectively. This SPME technique, applicable without using organic solvents, is simple and rapid. It is, therefore, useful for the practical detection of MA and AP in urine.