Citation

Campos LB, Pucca MB, Roncolato EC, Bertolini TB, Netto JC, Barbosa JE. Toxicon 2013; 76: 1-10.

Affiliation

Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil.

Copyright

(Copyright © 2013, Elsevier Publishing)

DOI

10.1016/j.toxicon.2013.08.063

PMID

23998940

Abstract

In various types of snake venom, the major toxic components are proteinases and members of the phospholipase A2 family, although other enzymes also contribute to the toxicity. In this study, we evaluated the proteolytic, phospholipase, and L-amino acid oxidase activities in the venom of five Bothrops species-B. jararaca, B. jararacussu, B. moojeni, B. neuwiedi, and B. alternatus-all of which are used in the production of commercial antivenom, prepared in horses. The enzymatic activities of each species' venom were classified as high, moderate, or low. B. moojeni venom demonstrated the highest enzymatic activity profile, followed by the venom of B. neuwiedi, B. jararacussu, B. jararaca, and B. alternatus. To our knowledge, this is the first study to compare all of these enzymes from multiple species, which is significant in view of the activity of L-amino acid oxidase across Bothrops species.

Language: en