Citation

Brandfellner HM, Ruparel SB, Gelfond JA, Hargreaves KM. Shock 2013; 40(3): 182-187.

Affiliation

1 Department of Surgery, University of Texas Health Science Center San Antonio 2 Department of Endodontics, University of Texas Health Science Center San Antonio 3 Department of Epidemiology and Biostatistics, University of Texas Health Science Center San Antonio.

Copyright

(Copyright © 2013, The Shock Society, Publisher Lippincott Williams and Wilkins)

DOI

10.1097/SHK.0b013e31829de02f

PMID

23817354

Abstract

: Tissue injury, such as burns or inflammation, can lead to the generation of oxidized lipids capable of regulating hemodynamic, pulmonary, immune and neuronal responses. However, it is not known whether traumatic injury leads to a selective up-regulation of transcripts encoding oxidative enzymes capable of generating these mediators. Here, we analyzed microarrays taken from circulating leukocytes of 187 trauma subjects compared to 97 control volunteers for changes in expression of 105 oxidative enzymes and related receptors. The results indicate that major blunt trauma triggers a selective change in gene expression with some transcripts undergoing highly significant up-regulation (e.g., CYP2C19), while others displaying significantly reduced expression (e.g., CYP2U1). This pattern in gene expression was maintained for up to 28 days after injury. In addition, the level of expression of CYP2A7, CYP2B7P1, CYP2C19, CYP2E1, CYP4A11, CYP4F3, CYP8B1, CYP19A1, CYP20A1, CYP51A1, HMOX2, NCF1, NCF2, NOX1, and the receptors PTGER2 and ESR2 were correlated with clinical trauma indices such as APACHE II, Max Denver Scale and the Injury Severity Score. Demonstration of a selective alteration in expression of transcripts encoding oxidative enzymes reveals a complex molecular response to major blunt trauma in circulating leukocytes. Further, the association between changes in gene expression and clinical trauma scores suggests an important role in integrating pathophysiologic responses to blunt force trauma.

Language: en