Citation

Senarathna SM, Ranganathan SS, Buckley N, Soysa SS, Fernandopulle BM. Indian J. Pharmacol. 2012; 44(4): 463-468.

Affiliation

Department of Pharmacology, Faculty of Medicine, University of Colombo, Sri Lanka ; South Asian Clinical Toxicology Research Collaboration, University of Sri Jayewardenepura, Sri Lanka ; Pharmacy Program, Department of Medical Education and Health Sciences, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka ; School of Pharmacy, Curtin University, Perth, Australia.

Copyright

(Copyright © 2012, Medknow Publications)

DOI

10.4103/0253-7613.99305

PMID

23087506

Abstract

OBJECTIVES: Acute paracetamol poisoning is an emerging problem in Sri Lanka. Management guidelines recommend ingested dose and serum paracetamol concentrations to assess the risk. Our aim was to determine the usefulness of the patient's history of an ingested dose of >150 mg/kg and paracetamol concentration obtained by a simple colorimetric method to assess risk in patients with acute paracetamol poisoning. MATERIALS AND METHODS: Serum paracetamol concentrations were determined in 100 patients with a history of paracetamol overdose using High Performance Liquid Chromatography (HPLC); (reference method). The results were compared to those obtained with a colorimetric method. The utility of risk assessment by reported dose ingested and colorimetric analysis were compared. RESULTS: The area under the receiver operating characteristic curve for the history of ingested dose was 0.578 and there was no dose cut-off providing useful risk categorization. Both analytical methods had less than 5% intra- and inter-batch variation and were accurate on spiked samples. The time from blood collection to result was six times faster and ten times cheaper for colorimetry (30 minutes, US$2) than for HPLC (180 minutes, US$20). The correlation coefficient between the paracetamol levels by the two methods was 0.85. The agreement on clinical risk categorization on the standard nomogram was also good (Kappa = 0.62, sensitivity 81%, specificity 89%). CONCLUSIONS: History of dose ingested alone greatly over-estimated the number of patients who need antidotes and it was a poor predictor of risk. Paracetamol concentrations by colorimetry are rapid and inexpensive. The use of these would greatly improve the assessment of risk and greatly reduce unnecessary expenditure on antidotes.

Language: en