Citation

Trachsel C, Siegemund D, Kämpfer U, Kopp LS, Bühr C, Grossmann J, Lüthi C, Cunningham M, Nentwig W, Kuhn-Nentwig L, Schürch S, Schaller J. FEBS J. 2012; 279(15): 2683-2694.

Affiliation

Department of Chemistry and Biochemistry, University of Bern, CH-3012 Bern, Switzerland Institute of Ecology and Evolution, University of Bern, CH-3012 Bern, Switzerland Functional Genomics Center Zürich, University of Zürich/ETH Zürich, CH-8057 Zürich, Switzerland.

Copyright

(Copyright © 2012, John Wiley and Sons)

DOI

10.1111/j.1742-4658.2012.08650.x

PMID

22672445

Abstract

The multi-component venom of the spider Cupiennius salei (C. salei) was separated by three different chromatographic strategies to facilitate subsequent analysis of peptidic venom components by tandem mass spectrometry (MALDI-TOF-MS and ESI-MS), Edman degradation, and amino acid analysis: 1) Desalting of the crude venom by RP-HPLC only, 2) Chromatographic separation of the crude venom into 42 fractions by RP-HPLC, and 3) Multi-dimensional purification of the crude venom by size exclusion- and cation exchange chromatography and RP-HPLC. A total of 286 components were identified in the venom of C. salei by mass spectrometry and the sequence of 49 new peptides was determined de novo by Edman degradation and tandem mass spectrometry, of which 30 were C-terminally amidated. The novel peptides were assigned to two main groups: 1) Short cationic peptides (SCPs) and 2) Cys-containing peptides with the inhibitor cystine knot (ICK) motif. Bioinformatics revealed a limited number of substantial similarities, namely with the peptides CpTx1 from the spider Cheiracantium punctorium and U3-ctenitoxin-Asp1a from the South American fishing spider (Ancylometes sp.) and with sequences from a Lycosa singoriensis venom gland transcriptome analysis. The results clearly indicate that the quality of the data is strongly dependent on the chosen separation strategy. The combination of orthogonal analytical methods efficiently excludes alkali ion and matrix adducts, provides indispensable information for an unambiguous identification of isomasses, and results in the most comprehensive repertoire of peptides identified in the venom of C. salei so far.

Language: en