Citation

Lemire SW, Ashley DL, Calafat AM. J. Anal. Toxicol. 2003; 27(1): 1-6.

Affiliation

Division of Laboratory Sciences, National Center for Environmental Health/Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. SGL4@cdc.gov

Copyright

(Copyright © 2003, Preston Publications)

DOI

unavailable

PMID

12587675

Abstract

Nitrogen mustards are a public health concern because of their extreme vesicant properties and the possible exposure of workers during the destruction of chemical stockpiles. A sensitive, rapid, accurate, and precise analysis for the quantitation of ultratrace levels of N-ethyldiethanolamine (EDEA) and N-methyldiethanolamine (MDEA) in human urine as a means of assessing recent exposure to the nitrogen mustards bis(2-chloroethyl)ethylamine and bis(2-chloroethyl)methylamine, respectively, was developed. The method was based on solid-phase extraction, followed by analysis of the urine extract using isotope-dilution high-performance liquid chromatography-mass spectrometry with TurbolonSpray ionization and multiple-reaction monitoring. The method limits of detection were 0.41 ng/mL for EDEA and 0.96 ng/mL for MDEA in 1 mL of urine with coefficients of variation < 10% for both compounds.

Language: en