Citation

Marques EP, Gil F, Proen'ca P, Monsanto P, Oliveira MF, Castanheira A, Vieira DN. Forensic Sci. Int. 2000; 110(2): 145-152.

Affiliation

Laborat¿orio de Toxicologia Forense, Instituto de Medicina Legal de Coimbra, Faculdade de Medicina da Universidade de Coimbra, Coimbra, Portugal. imltoxic@ci.uc.pt

Copyright

(Copyright © 2000, Elsevier Publishing)

DOI

unavailable

PMID

10808103

Abstract

This paper describes an analytical method for strychnine determination in biological samples by gas chromatography/mass spectrometry and their application in the investigation of two cases involving strychnine ingestion: A fatal case and a clinical one. The strychnine is isolated from biological samples using a liquid-liquid extraction procedure. The clean-up procedure is performed using an acid solution. Papaverine is used as internal standard in the quantification of strychnine. In the analysed specimens, the limits of quantification were 0.1 microg/ml or 0.1 microg/g. The recovery rate ranged from 75.0% to 98.7% and the coefficients of variation ranged from 4.8% to 10.5%.

Language: en