Citation

Luxembourg A, Hannaman D, Nolan E, Ellefsen B, Nakamura G, Chau L, Tellez O, Little S, Bernard R. Vaccine 2008; 26(40): 5216-5222.

Affiliation

Ichor Medical Systems, 6310 Nancy Ridge Drive, Ste 107, San Diego, CA 92121, USA. aluxembourg@verizon.net

Copyright

(Copyright © 2008, Elsevier Publishing)

DOI

10.1016/j.vaccine.2008.03.064

PMID

18462850

Abstract

DNA vaccines are a promising method of immunization against biothreats and emerging infections because they are relatively easy to design, manufacture, store and distribute. However, immunization with DNA vaccines using conventional delivery methods often fails to induce consistent, robust immune responses, especially in species larger than the mouse. Intramuscular (i.m.) delivery of a plasmid encoding anthrax toxin protective antigen (PA) using electroporation (EP), a potent DNA delivery method, rapidly induced anti-PA IgG and toxin neutralizing antibodies within 2 weeks following a single immunization in multiple experimental species. The delivery procedure is particularly dose efficient and thus favorable for achieving target levels of response following vaccine administration in humans. These results suggest that EP may be a valuable platform technology for the delivery of DNA vaccines against anthrax and other biothreat agents.

Language: en