Citation

Suntrarachun S, Pakmanee N, Tirawatnapong T, Chanhome L, Sitprija V. Toxicon 2001; 39(7): 1087-1090.

Affiliation

Department of Research and Development, Queen Saovabha Memorial Institute, Thai Red Cross Society, Rama IV Road, Patumwan, 10330, Bangkok, Thailand. sunutcha@hotmail.com

Copyright

(Copyright © 2001, Elsevier Publishing)

DOI

unavailable

PMID

11223099

Abstract

A PCR technique was used in this study to identify and distinguish monocellate cobra snake bites using snake venoms and swab specimens from snake bite-sites in mice from bites by other common Thai snakes. The sequences of nucleotide primers were selected for the cobrotoxin-encoding gene from the Chinese cobra (Naja atra) since the sequences of monocellate cobra (Naja kaouthia) venom are still unknown. However, the 113-bp fragment of cDNA of the cobrotoxin-encoding gene was detected in the monocellate cobra venom using RT-PCR. This gene was not found in the venoms of Ophiophagus hannah (king cobra), Bungarus fasciatus (banded krait), Daboia russelii siamensis (Siamese Russell's Viper, and Calloselasma rhodostoma (Malayan pit viper). Moreover, direct PCR could detect a 665-bp fragment of the cobrotoxin-encoding gene in the monocellate cobra venom but not the other snake venoms. Likewise, this gene was only observed in swab specimens from cobra snake bite-sites in mice. This is the first report demonstrating the ability of PCR to detect the cobrotoxin-encoding gene from snake venoms and swab specimens. Further studies are required for identification of this and other snakes from the bite-sites on human skin.

Language: en