Citation

Girón ME, Estrella A, Sánchez EE, Galán J, Tao WA, Guerrero B, Salazar AM, Rodriguez-Acosta A. Toxicon 2011; 57(4): 608-618.

Affiliation

Laboratorio de Inmunoquímica y Ultraestructura del Instituto Anatómico de la Universidad Central de Venezuela, Caracas, Venezuela, USA; Laboratorio de Inmunoquímica y Ultraestructura del Instituto Anatómico de la Universidad Central de Venezuela, Caracas, Venezuela, USA.

Copyright

(Copyright © 2011, Elsevier Publishing)

DOI

10.1016/j.toxicon.2011.01.003

PMID

21255600

PMCID

PMC3320208

Abstract

Porthidium lansbergii hutmanni is a small pit viper found on Margarita Island, Venezuela. Local tissue damage is one of the most obvious characteristics of P. l. hutmanni envenomation, which can lead to diverse pathological effects, such as hemorrhage, edema, blistering, necrosis, lymphatic vessel damage and degradation of extracellular matrix. Metalloproteinases are one of the major components in venoms responsible for these effects. To date, very little is known or has been reported on P. l. hutmanni venom. Crude P. l. hutmanni venom had a LD(50) of 2.5mg/kg and was considered very hemorrhagic (minimal hemorrhagic dose [MHD]: 0.98 μg) when compared to other hemorrhagic (Bothrops) venoms in Venezuela. Crude P. l. hutmanni venom also inhibited ADP-induced platelet aggregation. A metalloproteinase, Porthidin-1, from this venom was isolated by three chromatography steps (Sephadex G-100, Superose 12 HR10/30 and Bioscale Q2). Porthidin-1 falls in the SVMP P-I class having a molecular weight of 23kDa, verified by both SDS PAGE and mass spectrometry. High-resolution mass spectrometry and a database search identified a peptide from Porthidin-1 (YNGDLDK) belonging to the SVMP family of proteins. Porthidin-1 contained hemorrhagic, fibrino(geno)lytic, caseinolytic and gelatinolytic activities, and these activities were capable of being neutralized by metalloprotease inhibitors but not serine protease inhibitors. The peptide YNGDLDK shared similarities with five venom proteins with a BLAST e-value of<1. This work details the biochemical and pathophysiological effects that can result from envenomations, and highlights the importance and significance for characterizing unknown or poorly documented venoms from different geographical regions.

Language: en