CONTACT US: Contact info
|
Citation |
Goodall CR, Basteyns BJ. J. Anal. Toxicol. 1995; 19(6): 419-426. |
|
Affiliation |
Wisconsin State Laboratory of Hygiene, Department of Toxicology, Madison 53706, USA. |
|
Copyright |
(Copyright © 1995, Preston Publications) |
|
DOI |
unavailable |
PMID |
8926736 |
|
Abstract |
A sensitive and reliable method was developed for the identification and quantitation of cannabinoids in blood. Samples were screened by fluorescence polarization immunoassay. Analysis was completed on a benchtop mass selective detector using selected ion monitoring. The limits of detection were 0.2 ng/mL for delta9-tetrahydrocannabinol (THC and 11-hydroxy-THC and 2 ng/mL for 11-nor-9-carboxy-THC. Extensive method validation is presented, including within-run variation, between-run variation, and results from external proficiency testing. Sample stability was studied over a 6-month period. Several derivatives and extraction techniques were evaluated to determine optimum performance. Data from a blind study of 217 samples were used to determine the predictive value of the screening procedure. The procedure is used routinely in the laboratory on samples from drivers issued a citation for impaired driving and also on postmortem blood from death investigations. Language: en |