CONTACT US: Contact info
|
Citation |
Nadulski T, Bleeck S, Schräder J, Bork WR, Pragst F. Forensic Sci. Int. 2010; 196(1-3): 78-84. |
|
Affiliation |
Institute of Legal Medicine, University Hospital Charite, Hittorfstr. 18, 14195 Berlin, Germany. |
|
Copyright |
(Copyright © 2010, Elsevier Publishing) |
|
DOI |
|
PMID |
20074877 |
|
Abstract |
11-Nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid ethyl ester (THC-COOEt) can be presumed to be a mixed metabolite formed during combined consumption of cannabinoids and alcohol. In order to examine this hypothesis, THC-COOEt and its deuterated analogue D(3)-THC-COOEt were synthesized as reference substance and internal standard from the corresponding carboxylic acids and diazoethane and methods were developed for the sensitive detection of THC-COOEt in plasma and hair based on gas chromatography-electron impact mass spectrometry after silylation with N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide and gas chromatography-negative chemical ionization mass spectrometry (GC-NCI-MS) as well as tandem mass spectrometry (GC-NCI-MS-MS) after derivatization with pentafluoropropionyl anhydride. The methods were applied for THC-COOEt determination to plasma samples from 22 drunk driving cases which contained both ethanol (0.30-2.16mg/g) and THC-COOH (15-252ng/mL) as well as to 12 hair samples from drug fatalities which were both positive for THC (0.09-2.04ng/mg) and fatty acid ethyl esters as markers of chronic alcohol abuse (0.70-6.3ng/mg). In none of these samples THC-COOEt could be found with limits of detection of 0.3ng/mL in plasma and 2pg/mg in hair in 11 samples using GC-NCI-MS and 0.2pg/mg in one sample using GC-NCI-MS. Therefore, the use of this compound as a marker for combined cannabis and alcohol consumption could not be achieved. Language: en |