Citation

Mattie DR, Hoeflich TJ, Jones CE, Horton ML, Whitmire RE, Godin CS, Flemming CD, Andersen ME. Toxicol. Ind. Health 1993; 9(6): 995-1016.

Affiliation

Toxicology Division, Armstrong Laboratory, Wright-Patterson Air Force Base, OH 45433-7400.

Copyright

(Copyright © 1993, SAGE Publishing)

DOI

unavailable

PMID

8191505

Abstract

The subchronic (26 day) oral toxicities of two AF hydraulic fluids (MIL-H-5606 H5., MIL-H-83282 H8.), a commercial phosphate ester (PE), and two candidate hydraulic fluids (low temperature version of MIL-H-83282 LT. and chlorotrifluorethylene oligomers polyCTFE.) were compared in male F-344 rats. Oral dosing was used in order to quickly compare these fluids to PolyCTFE, the only fluid at the time to have been tested in a 90-day inhalation study. Rats were initially dosed with 1.0 g/kg/day of each fluid. H8 increased alkaline phosphatase (ALKP) while LT produced an anemia and leukocytosis. Exposure to H5 fluid resulted in lymphocytopenia and persistent diuresis. Due to their greater toxicity, resulting in lethality in the first dosing study, only 0.5 g/kg/day of PE and PolyCTFE were administered in the second study. Exposure to PE (0.5 g/kg) resulted in an anemia and decreases in BW (day 10 until day 25), spleen/BW ratio, blood urea nitrogen (BUN), and creatinine (CREAT). PolyCREAT (0.5 g/kg) decreased BW (day 11 to the end of the study) and testicular weight. PolyCTFE (0.5 g/kg) increased relative spleen weights, various clinical chemistry parameters, and triggered a reversible diuresis. PolyCTFE (0.5 g/kg), PE (0.5 g/kg), and H5 produced an increase in absolute and relative liver weights compared to control livers. Peroxisomal beta oxidation, an indicator of peroxisomal proliferation, was significantly increased above control levels in the livers of all rats except the PE (0.5 g/kg) group, where the increase was not significant. Hydrocarbon nephropathy, indicated by increased levels of hyaline droplets in kidney tubules, was severe in H5, mild in H8, LT, and PolyCTFE (0.5 g/kg), and minimal in PE (0.5 g/kg). The MIL-H-83282 fluids (H8 and LT) were the least toxic hydraulic fluids. PolyCTFE and PE were the most toxic, with H5 intermediate.

Language: en