TY  - JOUR
PY  - 2009//
TI  - Intentional poisoning cases of animals with anticholinesterase pesticide-carbofuran in Sri Lanka
JO  - Legal medicine (Elsevier)
A1  - Tennakoon, Sakunthala
A1  - Perera, Bilesha
A1  - Haturusinghe, Lathika
SP  - S500
EP  - S502
VL  - 11
IS  - 1
N2  - Carbofuran is a broad spectrum insecticide and nematicide which inhibits acetyl cholinesterase. Several intentional poisoning cases of animals and birds including crows, dogs, cow, and elephant, using carbofuran were reported in Sri Lanka. Qualitative analysis of carbofuran in biological specimens was carried out using T.L.C and GC-MS. The quantitative analysis was carried out by HPLC using Zorbax Eclips XDB-C18 (150x4.6mm I.Dx5mum particle size) column with acetonitrile: water 25:75v/v mobile phase and UV detection at 210nm [1]. The liquid-liquid extraction with chloroform was reproducible and sensitive. The procedure was validated in terms of linearity (0.996<r<0.999) for concentrations ranging from 1 to 200mug/ml, repeatability (RSD 4.4% at 100mug/ml, n=10) extraction recovery (91.7% at 100mug/g, 77.3% at 20mug/g), limit of detection (S/N ratio=3, 0.7mug/ml) and limit of quantification (S/N ratio=10, 2.4mug/ml). The method was found satisfactory and successfully applied in fatal cases of animals and birds due to carbofuran poisoning. The fatal carbofuran levels detected were in the range of 42-910mug/g in crow's gizzard and contents, 50-800mug/g in dog's stomach contents, 13-20mug/g in dog's liver, 0.9mug/g in cow's stomach and contents, 35mug/g in elephant's stomach and contents.<p />  <p>Language: en</p>
LA  - en
SN  - 1344-6223
UR  - http://dx.doi.org/10.1016/j.legalmed.2009.01.033
ID  - ref1
ER  -