TY  - JOUR
PY  - 2018//
TI  - Action of tyrosinase on caffeic acid and its n-nonyl ester. Catalysis and suicide inactivation
JO  - International journal of biological macromolecules
A1  - Garcia-Jimenez, Antonio
A1  - Teruel-Puche, Jose Antonio
A1  - García-Ruiz, Pedro Antonio
A1  - Saura-Sanmartin, Adrian
A1  - Berna, Jose
A1  - Rodriguez-Lopez, Jose Neptuno
A1  - García-Cánovas, Francisco
SP  - 2650
EP  - 2659
VL  - 107
IS  - Pt B
N2  - Different mechanisms for inhibiting tyrosinase can be designed to avoid postharvest quality losses of fruits and vegetables. The action of tyrosinase on caffeic acid and its n-nonyl ester (n-nonyl caffeate) was characterized kinetically in this work. The results lead us to propose that both compounds are suicide substrates of tyrosinase, for which we establish the catalytic and inactivation efficiencies. The ester is more potent as inactivator than the caffeic acid and the number of turnovers made by one molecule of the enzyme before its inactivation (r) is lower for the ester. We proposed that the anti-browning and antibacterial properties may be due to suicide inactivation processes.<p /> <p>Language: en</p>
LA  - en
SN  - 0141-8130
UR  - http://dx.doi.org/10.1016/j.ijbiomac.2017.10.151
ID  - ref1
ER  -