TY - JOUR PY - 2011// TI - Suicide inactivation of peroxidase from Chamaerops excelsa palm tree leaves JO - International journal of biological macromolecules A1 - Cuadrado, Nazaret Hidalgo A1 - Zhadan, Galina G. A1 - Roig, Manuel G. A1 - Shnyrov, Valery L. SP - 1078 EP - 1082 VL - 49 IS - 5 N2 - The concentration and time-dependences and the mechanism of the inactivation of Chamaerops excelsa peroxidase (CEP) by hydrogen peroxide were studied kinetically with four co-substrates (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), guaiacol, o-dianisidine and o-phenylenediamine). The turnover number (r) of H(2)O(2) required to complete the inactivation of the enzyme varied for the different substrates, the enzyme most resistant to inactivation (r=4844) with ABTS being the most useful substrate for biotechnological applications, opening a new avenue of enquiry with this peroxidase.
Language: en
LA - en SN - 0141-8130 UR - http://dx.doi.org/10.1016/j.ijbiomac.2011.09.001 ID - ref1 ER -