TY  - JOUR
PY  - 2008//
TI  - Peroxidase-like catalytic activity of aqueous- and immobilized-Mn(3+)-octabromo-porphyrins on ion-exchange resin supplied as mimetic of horseradish peroxidase
JO  - Chemical and pharmaceutical bulletin
A1  - Kitamura, Youji
A1  - Mori, Katsuya
A1  - Yamamoto, Makiko
A1  - Nozaki, Akira
A1  - Saito, Madoka
A1  - Tsukamoto, Ikuko
A1  - Mifune, Masaki
A1  - Saito, Yutaka
SP  - 1364
EP  - 1366
VL  - 56
IS  - 9
N2  - In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activity of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn(3+)-octabromotetrakis(1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.<p /> <p>Language: en</p>
LA  - en
SN  - 0009-2363
UR  - http://dx.doi.org/10.1248/cpb.56.1364
ID  - ref1
ER  -