TY  - JOUR
PY  - 1998//
TI  - Analysis of fenthion in postmortem samples by HPLC with diode-array detection and GC-MS using solid-phase extraction
JO  - Journal of analytical toxicology
A1  - Meyer, E.
A1  - Borrey, D.
A1  - Lambert, W.
A1  - Van Peteghem, C.
A1  - Piette, M.
A1  - De Leenheer, A.
SP  - 248
EP  - 252
VL  - 22
IS  - 3
N2  - Fenthion (O,O-dimethyl-O-[3-methyl-4-(methylthio)-phenyl]-thiophos-phate ) is an organophosphate insecticide. A specific method to quantitate fenthion in postmortem matrices with solid-phase extraction combined with high-performance liquid chromatography-diode-array detection (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS) is presented. Fenitrothion (O,O-dimethyl-O-[3-methyl-4-nitrophenyl]-thiophosphate) is selected as the internal standard. For sample cleanup, a simple but selective solid-phase extraction is chosen after comparison with traditional liquid-liquid extraction procedures. Homogenized and appropriately diluted aqueous samples are applied, and the analytes are desorbed with 5 mL of dichloromethane. Aliquots of the extract are used for HPLC-DAD and GC-MS analysis, Liquid and GC conditions are as follows: gradient elution with a mixture of methanol and water (10:90 to 90:10, v/v) containing 0.0125M NaOH on an Aluspher RP-Select B column monitoring at 250 nm, and temperature programming from 60 to 300 degrees C on a dimethylpolysiloxane column in the SCAN mode, respectively. This method is applied to a suicidal case involving unsuspected acute intoxication with fenthion (concentration in blood, 3.8 micrograms/mL).<p /> <p>Language: en</p>
LA  - en
SN  - 0146-4760
UR  - http://dx.doi.org/10.1093/jat/22.3.248
ID  - ref1
ER  -