TY  - JOUR
PY  - 1991//
TI  - Specific site distribution of TN 5 in pseudomonas putida PpGI-MI using suicide plasmid pSUP 2021
JO  - Egypt. J. Microbiol.
A1  - T.m.a., El Kawokgy
SP  - 443
EP  - 451
VL  - 26
IS  - 3
N2  - Transposon Tn 5, which confers kanamycin-neomycin resistance, was introduced into the genome of Pseudomonas putida PpG1-M1 by conjugation with Escherichia coli SM10 containing the suicide plasmid vector pSUP2021. Approximately, 1.82% of over 2200 neomycin resistant [Nmr] colonies were mutated to auxotrophy. Most auxotrophs reverted at low frequencies [<10-8] and most prototrophic revertants were neomycin sensitive. The Tn 5-induced auxotrophic mutations were mainly distributed among genes of aspartic acid group indicating specific site-distribution of Tn 5 among the genome of Pseudomonas putida PpG1-M1<p /> <p>Language: en</p>
LA  - en
SN  - 0022-2704
UR  - http://dx.doi.org/
ID  - ref1
ER  -