TY  - JOUR
PY  - 1991//
TI  - Estimation of kinetic parameters in the inactivation of an enzyme by a suicide substrate
JO  - Biochimica et biophysica acta
A1  - Funaki, T.
A1  - Takanohashi, Y.
A1  - Fukazawa, H.
A1  - Kuruma, I.
SP  - 43
EP  - 46
VL  - 1078
IS  - 1
N2  - A method was developed to estimate the extended Michaelis constant and maximum velocity of a suicide substrate from the time-course of remaining enzyme activity with the use of simulation data calculated from the representative kinetic model for a suicide substrate proposed by Walsh et al. (Walsh, C., Cromartie, T., Marcotte, P. and Spencer, R. (1978) Methods Enzymol. 53, 437-448). For this purpose an analytical equation for the time-course of remaining enzyme activity, based on the suicide kinetic model, was derived by the steady-state method reported by Tatsunami et al. (Tatsunami, S., Yago, N. and Hosoe, M. (1981) Biochim. Biophys. Acta 662, 226-235). The accuracy of this analytical solution was proved by comparing the result with the exact solution obtained by numerical computation. A method was also developed to estimate the most important factor for a suicide substrate, the partition ratio, from the time-course of remaining enzyme activity.<p /> <p>Language: en</p>
LA  - en
SN  - 0006-3002
UR  - http://dx.doi.org/10.1016/0167-4838(91)90090-m
ID  - ref1
ER  -