TY  - JOUR
PY  - 1993//
TI  - Suicide-substrate inactivation of beta-galactosidase by diazomethyl beta-D-galactopyranosyl ketone
JO  - Carbohydrate Research
A1  - BeMiller, J. N.
A1  - Gilson, R. J.
A1  - Myers, R. W.
A1  - Santoro, M. M.
SP  - 101
EP  - 112
VL  - 250
IS  - 1
N2  - Diazomethyl beta-D-galactopyranosyl ketone (1) has been proven to be a mechanism-based, irreversible (suicide-substrate) inactivator of Aspergillus oryzae beta-D-galactosidase, but not an inactivator of E. coli lacZ beta-D-galactosidase. Compound 1 is stable in buffers of normal physiological pH. It is decomposed by H+, but not by nucleophiles. Inactivation of A. oryzae beta-D-galactopyranosyl ketone (2) nor diazomethyl alpha-D-galactopyranosyl ketone inactivated the enzyme and therefore inactivation is stereospecific, excess inhibitor could be separated from inactive enzyme without regain of activity and therefore it is bound irreversibly, and a second pulse of enzyme is inactivated at the same rate as enzyme inactivated to 95% activity by the first pulse. Diazomethyl beta-D-glucopyranosyl ketone (2) inhibited sweet almond beta-D-glucosidase.<p /> <p>Language: en</p>
LA  - en
SN  - 0008-6215
UR  - http://dx.doi.org/10.1016/0008-6215(93)84159-4
ID  - ref1
ER  -