TY  - JOUR
PY  - 1995//
TI  - Endogenous interleukin 10 prevents apoptosis in macrophages during salmonella infection
JO  - Biochemical and biophysical research communications
A1  - Arai, T.
A1  - Hiromatsu, K.
A1  - Nishimura, H.
A1  - Kimura, Y.
A1  - Kobayashi, N.
A1  - Ishida, H.
A1  - Nimura, Y.
A1  - Yoshikai, Y.
SP  - 600
EP  - 607
VL  - 213
IS  - 2
N2  - To elucidate the biological roles of endogenous interleukin 10 (IL-10) in macrophage responses during bacterial infection, we examined in vitro effects of neutralizing IL-10 by anti-IL-10 monoclonal antibodies (mAb) on apoptosis of the peritoneal macrophages following Salmonella choleraesuis infection. Marked increments of TNF-α production were observed in the culture supernatant later than 6 h after in vitro culture with anti-IL-10 mAb. These macrophages succumbed to apoptosis at this stage accompanied by marked increment of IL-1 release, despite the expression of higher amount of endogenous heat shock protein 70, an inhibitor of TNF-α-mediated apoptosis. These results suggest that endogenous IL-10 plays an essential role in protection of Salmonella-infected macrophages from autocrine suicide caused by excessive production of TNF-α after killing of Salmonella. © 1995 by Academic Press, Inc.<p /> <p>Language: en</p>
LA  - en
SN  - 0006-291X
UR  - http://dx.doi.org/10.1006/bbrc.1995.2174
ID  - ref1
ER  -