TY  - JOUR
PY  - 1992//
TI  - Synthesis of an Oligonucleotide Suicide Substrate for DNA Methyltransferases
JO  - Journal of organic chemistry
A1  - MacMillan, A.M.
A1  - Chen, L.
A1  - Verdine, G.L.
SP  - 2989
EP  - 2991
VL  - 57
IS  - 11
N2  - The large-scale chemical synthesis of an oligodeoxynucleotide containing 5-fluoro-2′-deoxycytidine (FdC) and its characterization are described. The FdC residue is introduced via the corresponding 4-0-(2,4,6-trimethylphenyl)-2′-deoxyuridine derivative, which undergoes clean conversion to FdC during removal of the oligonucleotide protecting groups with ammonia. A double-stranded oligodeoxynucleotide containing FdC inactivated the DNA methyltransferase enzyme M.Hae Ⅲ by irreversible formation of a covalent protein-DNA complex. © 1992, American Chemical Society. All rights reserved.<p /> <p>Language: en</p>
LA  - en
SN  - 0022-3263
UR  - http://dx.doi.org/10.1021/jo00037a006
ID  - ref1
ER  -