TY  - JOUR
PY  - 2020//
TI  - A novel, robust method for quantification of multiple kynurenine pathway metabolites in the cerebrospinal fluid
JO  - Bioanalysis
A1  - Schwieler, Lilly
A1  - Trepci, Ada
A1  - Krzyzanowski, Stanislaw
A1  - Hermansson, Sigurd
A1  - Granqvist, Mathias
A1  - Piehl, Fredrik
A1  - Venckunas, Tomas
A1  - Brazaitis, Marius
A1  - Kamandulis, Sigitas
A1  - Lindqvist, Daniel
A1  - Jones, Arthur Daniel
A1  - Erhardt, Sophie
A1  - Brundin, Lena
SP  - 379
EP  - 392
VL  - 12
IS  - 6
N2  - AIM: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. <br><br>METHODology & results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. <br><br>CONCLUSION: The feasibility of reliably determining CSF TRP metabolites were demonstrated, including separation of the two isomers PIC and nicotinic acid (NA) and the finding of a reduced PIC/QUIN ratio replicated in suicide attempters.<p /> <p>Language: en</p>
LA  - en
SN  - 1757-6180
UR  - http://dx.doi.org/10.4155/bio-2019-0303
ID  - ref1
ER  -