TY  - JOUR
PY  - 2006//
TI  - Suicidal cancer vaccine enhances anti-tumor immunotherapeutic effect and its safety in the treatment of ovarian cancer
JO  - Chinese Journal of Oncology
A1  - Kang, Yu
A1  - Xu, Cong-jian
A1  - Liu, Xi-shi
A1  - Shao, Zhi-min
A1  - Ou, Zhou-luo
A1  - Luo, Jian-ming
A1  - Wu, Chao-qua
A1  - Zhong, Cui-ping
A1  - Gu, Jian-ren
SP  - 654
EP  - 657
VL  - 
IS  - 12
N2  - &lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To study the anti-tumor immunotherapeutic effect induced by the suicidalcancer vaccine FC/TK, and to evaluate the safety of this vaccine.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;The suicidal cancer vaccine, named FC/TK, was prepared by fusion of suicide gene (HSVI,-TK gene) -modified ovarian carcinoma NuTu-19 cells with rat bone marrow-derived dendritic cells (DCs). The morphology of FC/TK was evaluated by scanning electron microscopy. The stimulatory effect of FC/TK on T cells was determined by T cell proliferation assay. In immunotherapeutic studies in vivo, Fischer344 rats were injected subcutaneously with NuTu-19 cells, followed by treatment of FC/TK on days 7 and 14, compared to controls treated with irradiated FC/TK, FC or PBS, respectively. Tumor incidence and volume were measured in 90 days after challenge. To determine the killing effect of FC/TK in vivo, TUNEL assays were applied to detect apoptotic cell death in spleen of vaccinated rats with prodrug ganciclovir administration.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;FC/TK cells were of irregular shape with surface membrane processes. Compared to the control groups, FC/TK significantly promoted T cell proliferation (P &lt;0.01). The rats vaccinated with FC/TK and FC significantly inhibited the tumor growth compared to rats vaccinated with irradiated FC/TK (P &lt;0.05) or with PBS ( P &lt;0.01). The immunotherapeutic effect induced by FC/TK was similar to that using FC. Fluorescence microscopy showed that fluorescein-stained FC/TK cells migrated into spleen also showed to be TUNEL-positive, suggesting that the FC/TK cells were killed by ganciclovir in vivo.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;Our data indicate that suicidal cancer vaccine is an effective and safe therapy for ovarian carcinoma and may serve as a broadly applicable approach for other cancer vaccines in the future.&lt;/p&gt;<p /> <p>Language: zh</p>
LA  - zh
SN  - 0253-3766
UR  - http://dx.doi.org/
ID  - ref1
ER  -