TY  - JOUR
PY  - 2023//
TI  - Phenibut screening and quantification with liquid chromatography-tandem mass spectrometry and its application to driving cases
JO  - Journal of forensic sciences
A1  - Dziadosz, Marek
A1  - Rosenberger, Wolfgang
A1  - Bolte, Katarina
A1  - Klintschar, Michael
A1  - Teske, Jorg
SP  - ePub
EP  - ePub
VL  - ePub
IS  - ePub
N2  - An analytical strategy for identification by an LC-MS/MS multitarget screening method and a suitable LC-MS/MS based quantification were developed for the psychotropic drug phenibut. The samples analyzed were collected during traffic control and were associated with driving under the influence of drugs. A positive sample for phenibut was identified in a single case of driving under the influence. The quantification revealed a drug concentration of 1.9 μg/mL. An interaction with blood alcohol (BAC = 0.10%) was discussed as the explanation of the way of driving and deficit manifestations observed (swaying, nystagmus, quivering of the eyelid, and reddened eyes). According to the available information, the quantified phenibut concentration could be explained by an intake of four tablets (self-reported) during the day containing 250 mg of the drug. Chromatography was performed with a Luna 5 μm C18 (2) 100 A, 150 mm × 2 mm analytical column, and a buffer system consisted of 10 mM ammonium acetate and 0.1% acetic acid (v/v) included in mobile phases marked as A (H(2) O/methanol = 95/5, v/v) and B (H(2) O/methanol = 3/97, v/v). An effective limit of detection (LOD = 0.002 μg/mL) could be achieved for the multitarget screening method. The quantification of phenibut was performed on a second LC-MS/MS system with LOD/LOQ values of 0.22/0.40 μg/mL. Since phenibut quantification data are rare, the presented information can be used with caution for evaluation of positive cases in the future.    Keywords: Drug impaired driving <p />  <p>Language: en</p>
LA  - en
SN  - 0022-1198
UR  - http://dx.doi.org/10.1111/1556-4029.15455
ID  - ref1
ER  -