TY  - JOUR
PY  - 2023//
TI  - Transcriptome analysis reveals the peptide toxins diversity of Macrothele palpator venom
JO  - International journal of biological macromolecules
A1  - Xiao, Xin
A1  - Luo, Xiaoqing
A1  - Huang, Cuiling
A1  - Feng, Xujun
A1  - Wu, Meijing
A1  - Lu, Minjuan
A1  - Kuang, Jiating
A1  - Peng, Siyi
A1  - Guo, Yingmei
A1  - Zhang, Zixuan
A1  - Hu, Zhaotun
A1  - Zhou, Xi
A1  - Chen, Minzhi
A1  - Liu, Zhonghua
SP  - ePub
EP  - ePub
VL  - ePub
IS  - ePub
N2  - Spider venom is a large pharmacological repertoire of different bioactive peptide toxins. However, obtaining crude venom from some spiders is challenging. Thus, studying individual toxins through venom purification is a daunting task. In this study, we constructed the cDNA library and transcriptomic sequencing from a pair of Macrothele palpator venom glands. Subsequently, 718 high-quality expressed sequence tags (ESTs) were identified, and grouped into three categories, including 449 toxin-like (62.53 %), 136 cellular component (18.94 %) and 133 non-matched (18.52 %) based on the gene function annotation. Additionally, 112 non-redundant toxin-like peptides were classified into 13 families (families A-M) based on their sequence homology and cysteine framework. Bioinformatics analysis revealed a high sequence similarity between families A-J and the toxins from Macrothele gigas in the NR database. In contrast, families K-M had a generally low sequence homology with known spider peptide toxins and unpredictable biological functions. Taken together, this study adds many new members to the spider toxin superfamily and provides a basis for identifying various potential biological tools in M. palpator venom.<p />  <p>Language: en</p>
LA  - en
SN  - 0141-8130
UR  - http://dx.doi.org/10.1016/j.ijbiomac.2023.126577
ID  - ref1
ER  -