TY  - JOUR
PY  - 1993//
TI  - Incidence of psychoactive cannabinoids in drivers killed in motor vehicle accidents
JO  - Journal of forensic sciences
A1  - Gerostamoulos, Jim
A1  - Drummer, O. H.
SP  - 649
EP  - 656
VL  - 38
IS  - 3
N2  - A high performance liquid chromatography (HPLC) assay was developed for the psychoactive cannabinoids Delta-9-Tetrahydrocannabinol (THC) and 11-Hydroxy-THC (11-OH-THC) using electrochemical detection (ECD). A C8 bonded column was used and the mobile phase consisted of acetonitrile, methanol and 0.01 M sulphuric acid at a flow rate of 1.5 mL/min. The detection limits for both THC and 11-OH-THC were 1.0 ng/mL. Preliminary screening of 193 drivers using an enzyme-multiplied immunoassay technique (EMIT) showed 21 tested positive on either blood, urine or both. Of these subjects 13 were confirmed as positive by the HPLC/ECD method in blood. Blood concentration for THC ranged from 1.4 ng/mL up to 20 ng/mL and for 11-OH-THC 2.5 ng/mL up to 85 ng/mL.<p />  <p>Language: en</p>
LA  - en
SN  - 0022-1198
UR  - http://dx.doi.org/
ID  - ref1
ER  -