TY  - JOUR
PY  - 2021//
TI  - A rapid assay provides on-site quantification of tetrahydrocannabinol in oral fluid
JO  - Science translational medicine
A1  - Yu, Hojeong
A1  - Lee, Hoyeon
A1  - Cheong, Jiyong
A1  - Woo, Sang Won
A1  - Oh, Juhyun
A1  - Oh, Hyun-Kyung
A1  - Lee, Jae-Hyun
A1  - Zheng, Hui
A1  - Castro, Cesar M.
A1  - Yoo, Yeong-Eun
A1  - Kim, Min-Gon
A1  - Cheon, Jinwoo
A1  - Weissleder, Ralph
A1  - Lee, Hakho
SP  - eabe2352
EP  - eabe2352
VL  - 13
IS  - 616
N2  - Tetrahydrocannabinol (THC), the primary psychoactive ingredient of cannabis, impairs cognitive and motor function in a concentration-dependent fashion. Drug testing is commonly performed for employment and law enforcement purposes; however, available tests produce low-sensitive binary results (lateral flow assays) or have long turnaround (gas chromatography-mass spectrometry). To enable on-site THC quantification in minutes, we developed a rapid assay for oral THC analysis called EPOCH, express probe for on-site cannabis inhalation. EPOCH features distinctive sensor design such as a radial membrane and transmission optics, all contained in a compact cartridge. This integrated approach permitted assay completion within 5 minutes with a detection limit of 0.17 ng/mL THC, which is below the regulatory guideline (1 ng/mL). As a proof of concept for field testing, we applied EPOCH to assess oral fluid samples from cannabis users (n = 43) and controls (n = 43). EPOCH detected oral THC in all specimens from cannabis smokers (median concentration, 478 ng/mL) and THC-infused food consumers. Longitudinal monitoring showed a fast drop in THC concentrations within the first 6 hours of cannabis smoking (half-life, 1.4 hours)., The express probe for on-site cannabis inhalation (EPOCH) system is able to quantitates tetrahydrocannabinol concentrations in oral fluid.<p />  <p>Language: en</p>
LA  - en
SN  - 1946-6234
UR  - http://dx.doi.org/10.1126/scitranslmed.abe2352
ID  - ref1
ER  -