TY  - JOUR
PY  - 2021//
TI  - Advantages of filtration method for sperm-DNA genotyping in sexual assault cases
JO  - Legal medicine (Elsevier)
A1  - Nakagawa, Toshifumi
A1  - Doi, Masanori
A1  - Nishi, Kosuke
A1  - Sugahara, Takuya
SP  - e101988
EP  - e101988
VL  - 54
IS  - 
N2  - Differential extraction (DE) is a conventional method to isolate sperms from forensic semen samples (e.g. vaginal swab containing semen) for sperm-DNA genotyping. Subsequent to selective digestion of somatic cells in a mixture sample, sperms are collected and purified as a pellet by repetitive centrifugation based on the specific gravity of sperm heads. However, the centrifugation operation requires a technical proficiency and an extensive time to prevent a loss of sperms from the pellet as much as possible. Therefore, we devised a "filtration method (FM)", in which a vacuum filtration operation based on the size of sperm heads is adapted, instead of DE, for isolation of sperms without any loss in principle. Sperms are collected and purified on a polycarbonate membrane filter. In this study, we have compared results of forensic assays by DE and FM for sperm-DNA genotyping from forensic semen samples. Consequently, FM had advantages of easy operation, timesaving, and high yield of sperms from semen samples compared with DE, although FM had a comparable ability to DE for a purification of sperms from mixture samples. Thus, we present that FM could simply lead to success of sperm-DNA genotyping and has a possibility to supersede DE as a gold-standard method.<p />  <p>Language: en</p>
LA  - en
SN  - 1344-6223
UR  - http://dx.doi.org/10.1016/j.legalmed.2021.101988
ID  - ref1
ER  -