TY  - JOUR
PY  - 2021//
TI  - A variable height microfluidic device for multiplexed immunoassay analysis of traumatic brain injury biomarkers
JO  - Biosensors (Basel)
A1  - Burns, Mark A.
A1  - Korley, Frederick K.
A1  - Krausz, Alyse D.
SP  - e320
EP  - e320
VL  - 11
IS  - 9
N2  - Traumatic brain injury (TBI) is a leading cause of global morbidity and mortality, partially due to the lack of sensitive diagnostic methods and efficacious therapies. Panels of protein biomarkers have been proposed as a way of diagnosing and monitoring TBI. To measure multiple TBI biomarkers simultaneously, we present a variable height microfluidic device consisting of a single channel that varies in height between the inlet and outlet and can passively multiplex bead-based immunoassays by trapping assay beads at the point where their diameter matches the channel height. We developed bead-based quantum dot-linked immunosorbent assays (QLISAs) for interleukin-6 (IL-6), glial fibrillary acidic protein (GFAP), and interleukin-8 (IL-8) using Dynabeads(TM) M-450, M-270, and MyOne(TM), respectively. The IL-6 and GFAP QLISAs were successfully multiplexed using a variable height channel that ranged in height from ~7.6 µm at the inlet to ~2.1 µm at the outlet. The IL-6, GFAP, and IL-8 QLISAs were also multiplexed using a channel that ranged in height from ~6.3 µm at the inlet to ~0.9 µm at the outlet. Our system can keep pace with TBI biomarker discovery and validation, as additional protein biomarkers can be multiplexed simply by adding in antibody-conjugated beads of different diameters.<p />  <p>Language: en</p>
LA  - en
SN  - 2079-6374
UR  - http://dx.doi.org/10.3390/bios11090320
ID  - ref1
ER  -