TY  - JOUR
PY  - 2021//
TI  - Plasma mir-9-3p and mir-136-3p as potential novel diagnostic biomarkers for experimental and human mild traumatic brain injury
JO  - International journal of molecular sciences
A1  - Das Gupta, Shalini
A1  - Ciszek, Robert
A1  - Heiskanen, Mette
A1  - Lapinlampi, Niina
A1  - Kukkonen, Janne
A1  - Leinonen, Ville
A1  - Puhakka, Noora
A1  - Pitkänen, Asla
SP  - e1563
EP  - e1563
VL  - 22
IS  - 4
N2  - Noninvasive, affordable circulating biomarkers for difficult-to-diagnose mild traumatic brain injury (mTBI) are an unmet medical need. Although blood microRNA (miRNA) levels are reportedly altered after traumatic brain injury (TBI), their diagnostic potential for mTBI remains inconclusive. We hypothesized that acutely altered plasma miRNAs could serve as diagnostic biomarkers both in the lateral fluid percussion injury (FPI) model and clinical mTBI. We performed plasma small RNA-sequencing from adult male Sprague-Dawley rats (n = 31) at 2 days post-TBI, followed by polymerase chain reaction (PCR)-based validation of selected candidates. miR-9a-3p, miR-136-3p, and miR-434-3p were identified as the most promising candidates at 2 days after lateral FPI. Digital droplet PCR (ddPCR) revealed 4.2-, 2.8-, and 4.6-fold elevations in miR-9a-3p, miR-136-3p, and miR-434-3p levels (p < 0.01 for all), respectively, distinguishing rats with mTBI from naïve rats with 100% sensitivity and specificity. DdPCR further identified a subpopulation of mTBI patients with plasma miR-9-3p (n = 7/15) and miR-136-3p (n = 5/15) levels higher than one standard deviation above the control mean at <2 days postinjury. In sTBI patients, plasma miR-9-3p levels were 6.5- and 9.2-fold in comparison to the mTBI and control groups, respectively. Thus, plasma miR-9-3p and miR-136-3p were identified as promising biomarker candidates for mTBI requiring further evaluation in a larger patient population.<p />  <p>Language: en</p>
LA  - en
SN  - 1661-6596
UR  - http://dx.doi.org/10.3390/ijms22041563
ID  - ref1
ER  -