TY  - JOUR
PY  - 2020//
TI  - Sex does not influence visual outcomes after blast-mediated traumatic brain injury but IL-1 pathway mutations confer partial rescue
JO  - Investigative ophthalmology and visual science
A1  - Evans, Lucy P.
A1  - Boehme, Nickolas
A1  - Wu, Shu
A1  - Burghardt, Elliot L.
A1  - Akurathi, Abhigna
A1  - Todd, Brittany P.
A1  - Newell, Elizabeth A.
A1  - Ferguson, Polly J.
A1  - Mahajan, Vinit B.
A1  - Dutca, Laura M.
A1  - Harper, Matthew M.
A1  - Bassuk, Alexander G.
SP  - e7
EP  - e7
VL  - 61
IS  - 12
N2  - PURPOSE: In a mouse model of blast-mediated traumatic brain injury (bTBI), interleukin-1 (IL-1)-pathway components were tested as potential therapeutic targets for bTBI-mediated retinal ganglion cell (RGC) dysfunction. Sex was also evaluated as a variable for RGC outcomes post-bTBI.     Methods: Male and female mice with null mutations in genes encoding IL-1α, IL-1β, or IL-1RI were compared to C57BL/6J wild-type (WT) mice after exposure to three 20-psi blast waves given at an interblast interval of 1 hour or to mice receiving sham injury. To determine if genetic blockade of IL-1α, IL-1β, or IL-1RI could prevent damage to RGCs, the function and structure of these cells were evaluated by pattern electroretinogram and optical coherence tomography, respectively, 5 weeks following blast or sham exposure. RGC survival was also quantitatively assessed via immunohistochemical staining of BRN3A at the completion of the study.     Results: Our results showed that male and female WT mice had a similar response to blast-induced retinal injury. Generally, constitutive deletion of IL-1α, IL-1β, or IL-1RI did not provide full protection from the effects of bTBI on visual outcomes; however, injured WT mice had significantly worse visual outcomes compared to the injured genetic knockout mice.     Conclusions: Sex does not affect RGC outcomes after bTBI. The genetic studies suggest that deletion of these IL-1 pathway components confers some protection, but global deletion from birth did not result in a complete rescue.<p />  <p>Language: en</p>
LA  - en
SN  - 0146-0404
UR  - http://dx.doi.org/10.1167/iovs.61.12.7
ID  - ref1
ER  -