TY  - JOUR
PY  - 2019//
TI  - Comparison of different analytical methods for the determination of carbon monoxide in postmortem blood
JO  - Journal of forensic sciences
A1  - Zanaboni, Moira
A1  - Roda, Gabriella
A1  - Arnoldi, Sebastiano
A1  - Casagni, Eleonora
A1  - Gambaro, Veniero
A1  - Dei Cas, Michele
SP  - ePub
EP  - ePub
VL  - ePub
IS  - ePub
N2  - The determination of carbon monoxide (CO) and carboxyhemoglobin (COHb) is of utmost importance in forensic toxicology to determine the cause of death in cases of CO poisoning, fire, and explosions. To this end, reliable and updated analytical methods are required. In this paper, four different methods for the determination of carbon monoxide in postmortem blood samples were compared: (i) the spectrophotometric determination of COHb applying the method proposed by Rodkey and modified by Beutler-West, (ii) the spectrophotometric determination of CO using a micro-diffusion-based method, (iii) the determination of CO by gas chromatography coupled to a TCD detector, and (iv) the determination of COHb by blood gas analysis. Three postmortem blood samples were analyzed with all methods, and the results were comparable. The applied methodologies showed different features depending on the sensitivity, sample preparation, and volume. The HS-GC/TCD method in our hand was the most appropriate, on postmortem samples, and versatile to apply. Unfortunately, only a limited number of postmortem blood samples were available for this study due to the rarity of that kind of intoxication in our jurisdiction.<br><br>© 2019 American Academy of Forensic Sciences.<p />  <p>Language: en</p>
LA  - en
SN  - 0022-1198
UR  - http://dx.doi.org/10.1111/1556-4029.14206
ID  - ref1
ER  -